Li, Hao

Address：No. 15 Beisanhuan east road, Chaoyang 

Education background:

1995-1999, Department of Microbiology, Shandong University, B.S

1999-2002, College of Life Science, Shandong University, M.S

2002-2005, College of Marine Biology, China Ocean University, Ph.D

Work experience :

2005-2008, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Postdoctoral fellow

2008-Present, College of Life Science and Technology, Beijing University of Chemical Technology, Associate Professor

Academic title: 

Research areas: 

1) Microbiology.

Includes stress tolerance of microbial cells, and microbial interaction.

2) Selection, optimization and application of aptamer.

Includes proposing novel strategy for selection, optimization and application of aptamer; and development aptamer based biosensors for diagnosing clinical biomarkers, detecting drug residues in food, and monitoring environmental pollutants.

3) System biology and its application.

Honors and awards:

2018, Top ten teachers of Beijing University of Chemical Engineering

Research projects:

In recent five years, as the project host, I have undertaken one National Natural Science Foundation project, one national 863 major project sub project, one class B special cooperation project of Chinese Academy of Sciences, one marine high-tech industry development project of Fujian Province, one independent innovation and achievement transformation project of Shandong Province, two National Key Laboratory Open Fund projects and one enterprise entrusted project.

Representative works:

1)     Yujing Ding, Zihan Gao, Hao Li*. Real milk sample assisted selection of specific aptamer towards sarafloxacin and its application in establishment of an effective aptasensor. Sensors and Actuators B: Chemical, 2021, 343: 130113. (*Corresponding author) (TOP)

2)     Xin Kang, Zihan Gao, Lingjie Zheng, Xiaoru Zhang, Hao Li*. Regulation of Lactobacillus plantarum on the reactive oxygen species related metabolisms of Saccharomyces cerevisiae. LWT-Food Science and Technology, 2021, 147, 111492.(*Corresponding author) (TOP)

3)     Zihan Gao, Xiaoyan Du, Yujing Ding, Hao Li*. Establishment of a dual-aptasensor for simultaneous detection of chloramphenicol and kanamycin. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment, 2021, DOI: 10.1080/19440049.2021.1914871. (*Corresponding author)

4)     Xianlin He, Bo Liu, Yali Xu, Ze Chen, Hao Li*. Effects of Lactobacillus plantarum on the ethanol tolerance of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 2021, 105(6), 2597-2611. DOI: 10.1007/s00253-021-11198-x. (*Corresponding author) (TOP)

5)     Hongwei Hu, Yujing Ding, Zihan Gao, Hao Li*. S1 nuclease digestion-based rational truncation of PD-L1 aptamer and establishment of a signal dual amplification aptasensor.Sensors and Actuators B: Chemical, 2021, 331: 129442. (*Corresponding author) (TOP)

6)     Xiaoru Zhang, Yaxian Zhang, Zhihua Ji, Fengbang Wang, Lei Zhang, Maoyong Song*, Hao Li*. Oxidative damage mechanism in Saccharomyces cerevisiae cells exposed to tetrachlorobisphenol A. Environmental Toxicology and Pharmacology, 2020, 80: 103507. (*Corresponding author)

7)     Yujing Ding, Yuanpu Niu, Ze Chen, Shijun Dong, Hao Li*. Discovery of novel Lactobacillus plantarum co-existence-associated influencing factor(s) on Saccharomyces cerevisiae fermentation performance. LWT-Food Science and Technology, 2020, 135: 110268. (*Corresponding author) (TOP)

8)     Xiaoru Zhang, Yaxian Zhang, Hao Li*. Regulation of trehalose, a typical stress protectant, on central metabolisms, cell growth and division of Saccharomyces cerevisiae CEN.PK113-7D. Food Microbiology, 2020, 89: 103459. (*Corresponding author) (TOP)

9)     Chuchu Wang, Xiaoyan Du, Tiantian Xie, Hao Li*. Label- and modification-free-based in situ selection of bovine serum albumin specific aptamer. Journal of Separation Science, 2019, 42 (23): 3571-3578. (*Corresponding author)

10)   Ming Li, Hongwei Hu, Ze Chen, Yaxian Zhang, Hao Li*. Using drug-loaded pH-responsive poly(4-vinylpyridine) microspheres as a new strategy for intelligent controlling of Lactobacillus plantarum contamination in bioethanol fermentation. World Journal of Microbiology and Biotechnology, 2018, 34 (10): 146. (*Corresponding author)

11)   Ze Chen, Zhou Zheng, Fenglian Wang, Yuanpu Niu, Jinlai Miao*, Hao Li*. Intracellular metabolic changes of Rhodococcus sp. LH during the biodegradation of diesel oil. Marine Biotechnology, 2018, 20 (6): 803-812. (*Corresponding author) (TOP)

12)   Zhihua Ji, Yaxian Zhang, Juan Tian, Fengbang Wang, Maoyong Song*, Hao Li*. Oxidative stress and cytotoxicity induced by tetrachlorobisphenol A in Saccharomyces cerevisiae cells. Ecotoxicology and Environmental Safety, 2018, 161: 1-7. (*Corresponding author) (TOP)

13)   Lihui Wang, Chuchu Wang, Hao Li*. Selection of DNA aptamers and establishment of an effective aptasensor for highly sensitive detection of cefquinome residues in milk. Analyst, 2018, 143: 3202-3208. (*Corresponding author)

14)   Juan Tian, Zhihua Ji, Fengbang Wang, Maoyong Song*, Hao Li*. The toxic effects of Tetrachlorobisphenol A in Saccharomyces cerevisiae cells via metabolic interference. Scientific Reports, 2017, 7 (1): 2655. (*Corresponding author)

15)   Ye Duan, Lihui Wang, Zhiqiang Gao, Huishan Wang, Hexiao Zhang*, Hao Li*. An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR. Talanta, 2017, 165: 671-676. (*Corresponding author) (TOP)

16)   Bo Liu,Huaqing Liu, Yunxiao Zhang, Hao Li*. Control of Lactobacillus plantarum contamination in bioethanol fermentation by adding plantaricins. International Journal of Agriculture and Biology, 2017, 19: 171-176. (*Corresponding author)

17)   Juan Tian, Shuxian Zhang, Hao Li*. Changes in intracellular metabolisms underlying the adaptation of Saccharomyces cerevisiae strains to ethanol stress. Annals of Microbiology, 2017, 67 (2): 195-202. (*Corresponding author)

18)   Ze Chen, Zhou Zheng, Chenfeng Yi, Fenglian Wang, Yuanpu Niu, Hao Li*.Intracellular metabolic changes of Saccharomyces cerevisiaeand promotion to ethanol tolerance during the bioethanol fermentation process. RSC Advances, 2016, 6: 105046-105055. (*Corresponding author)

19)   Yuanpu Niu, Xianghua Lin, Shijun Dong, Qipeng Yuan, Hao Li*. Indentation with atomic force microscope,Saccharomyces cerevisiae cell gains elasticity under ethanol stress. The International Journal of Biochemistry & Cell Biology, 2016, 79: 337-344. (*Corresponding author)

20)   Yanfeng Wang, Juan Tian, Zhihua Ji, Maoyong Song, Hao Li*. Intracellular metabolic changes of Clostridium acetobutylicum and promotion to butanol tolerance during biobutanol fermentation. The International Journal of Biochemistry & Cell Biology, 2016, 78: 297-306.  (*Corresponding author)

21)   Ye Duan, Zhiqiang Gao, Lihui Wang, Huishan Wang, Hexiao Zhang*, Hao Li*. Selection and identification of chloramphenicol specific DNA aptamers by Mag-SELEX. Applied Biochemistry and Biotechnology, 2016, 180 (8): 1644-1656. (*Corresponding author)

22)   Chenfeng Yi, Fenglian Wang, Shijun Dong, Hao Li*. Changes of trehalose content and expression of relative genes during the bioethanol fermentation by Saccharomyces cerevisiae. Canadian Journal of Microbiology, 2016, 62 (10): 827-835. (*Corresponding author)

23)   Shijun Dong, Chenfeng Yi, Hao Li*. Changes of Saccharomyces cerevisiae cell membrane components and promotion to ethanol tolerance during the bioethanol fermentation. The International Journal of Biochemistry & Cell Biology, 2015, 69: 196-203. (*Corresponding author)

24)    Shijun Dong, Xianghua Lin, Hao Li*. Regulation of Lactobacillus plantarum contamination on the carbohydrate and energy related metabolisms of Saccharomyces cerevisiae during bioethanol fermentation. The International Journal of Biochemistry & Cell Biology, 2015, 68: 33-41. (*Corresponding author)

25)    Xianghua Lin, Pei Han, Shijun Dong, Hao Li*.Preparation and application of bacteriophage-loaded chitosan microspheres for controlling Lactobacillus plantarum contamination in bioethanol fermentation. RSC Advances, 2015, 5: 69886-69893. (*Corresponding author)

26)   Yanfeng Wang, Shuxian Zhang, Huaqing Liu, Lei Zhang, Chenfeng Yi, Hao Li*. Changes and roles of membrane compositions in the adaptation of Saccharomyces cerevisiae to ethanol.Journal of Basic Microbiology, 2015, 55 (12): 1417-1426. (*Corresponding author)

27)   Fengxia Cui, Ruimin Zhang, Huaqing Liu, Yanfeng Wang, Hao Li*. Metabolic responses to Lactobacillus plantarum contamination or bacteriophage treatment in Saccharomyces cerevisiae using a GC-MS-based metabolomics approach. World Journal of Microbiology & Biotechnology, 2015, 31 (12): 2003-2013.(*Corresponding author)

28)    Fengxia Cui, Huaqing Liu, Zhongmei Zou*,Hao Li*. Metabolic responses to water deprivation in C57BL/6J mice using a proton nuclear magnetic resonance-based metabonomics approach. RSC Advances, 2015, 5: 80142-80149. (*Corresponding author)

29)    Ribang Wu, CuiLing Wu, Dan Liu, Xinghao Yang, Jiafeng Huang, Jiang Zhang, Binqiang Liao, Hailun He*, Hao Li*. Overview of antioxidant peptides derived from marine resources: the sources, characteristic, purification, and evaluation methods. Applied Biochemistry and Biotechnology, 2015,176 (7): 1815-1833. (*Corresponding author)

30)    Dan Liu, Xinghao Yang, Jiafeng Huang, Ribang Wu, Cuiling Wu, Hailun He*, Hao Li*. In situ demonstration and characteristic analysis of the protease components from marine bacteria using substrate immersing zymography. Applied Biochemistry and Biotechnology, 2015, 175 (1): 489-501. (*Corresponding author)

31)   Manli Ma, Pei Han, Ruimin Zhang, Hao Li*. Ultrastructural changes of Saccharomyces cerevisiae in response to ethanol stress. Canadian Journal of Microbiology, 2013, 59 (9): 589-597. (*Corresponding author)

32)    Xianmei Hu, Hao Li*, Pingwah Tang, Jie Sun, Qipeng Yuan, Chunfang Li. GC-MS-based metabolomics study of the responses to arachidonic acid in Blakeslea trispora. Fungal Genetics and Biology, 2013, 57: 33-41. (*Co-first author)

33)    Hao Li, Manli Ma, Sha Luo, Ruimin Zhang, Pei Han, Wei Hu. Metabolic responses to ethanol in Saccharomyces cerevisiae using a gas chromatography tandem spectrometry-based metabolomics approach. The International Journal of Biochemistry & Cell Biology, 2012, 44 (7): 1087-1096.

34)    Hao Li, Manli Ma, Huijun Xie, Jian Kong. Biosafety evaluation of bacteriophages for treatment of diarrhea due to intestinal pathogen Escherichia coli 3-2 infection of chickens. World Journal of Microbiology and Biotechnology, 2012, 28: 1-6.

35)    Hao Li, Lan Wang, Xianzhong Yan, Qijun Liu, Chaohui Yu, Handong Wei, Youming Li, Xuemin Zhang, Fuchu He, Ying Jiang. A proton nuclear magnetic resonance metabonomics approach for biomarker discovery in nonalcoholic fatty liver disease. Journal of Proteome Research, 2011, 10 (6): 2797-2806.

36)    Zijun He, Shizeng Wang, Yumeng Yang, Jing Hu, Can Wang, Hao Li, Bokai Ma, Qipeng Yuan. β-Carotene production promoted by ethylene in Blakeslea trispora and the mechanism involved in metabolic responses. Process Biochemistry, 2017, 57: 57-63.

37)    Ming Yang, Meng Ren, Yue Qu, Wendi Teng, Zhongpeng Wang, Hao Li, Qipeng Yuan. Sulforaphene inhibits hepatocellular carcinoma through repressing keratin 8 and activating anoikis. RSC Advances, 2016, 6 (74): 70326-70334.

38)    Ge Wang, Zehao Li, Hao Li, LidanLi, Jian Li, Changyuan Yu. Metabolic profile changes of CCl4-liver fibrosis and inhibitory effects of Jiaqi Ganxian granule. Molecules, 2016, 21 (6). pii: E698.

39)    Jing Hu, Hao Li, Yumeng Yang, Shizeng Wang, Pingwah Tang, Chunfang Li, Guifang Tian, Qipeng Yuan. Metabolic regulation of α-linolenic acid on β-carotene synthesis in Blakeslea trispora revealed by a GC-MS-based metabolomic approach. RSC Advances, 2015, 5 (78): 63193-63201.

40)    Shizeng Wang, Hao Li, Xiaoguang Fan, Jingkun Zhang, Pingwah Tang, Qipeng Yuan. Metabolic responses in Candida tropicalis to complex inhibitors during xylitol bioconversion. Fungal Genetics and Biology, 2015, 82: 1-8.

Admission requirements:

High practical ability, active thinking, and broad vision.